Annual Report 2004

I. Outline of the Noda Institute for Scientific Research

II. Achievement of Research Activities in 2004

Reproduced Papers

T. Takahashi, O. Hatamoto, Y. Koyama and K. Abe: Efficient gene disruption in the koji-mold Aspergillus sojae using a novel variation of the positive-negative method
　Reproduced from Mol Gen Genomics, 272: 344-352 (2004) by copyright permission of the Springer-Verlag
A. Ventosa, M. C. Gutiérrez, M. Kamekura, I. S. Zvyagintseva and A. Oren: Taxonomic study of Halorubrum distributum and proposal of Halorubrum terrestre sp. nov.
　Reproduced from Int. J. Syst. Evol. Microbiol., 54: 389-392 (2004) by copyright permission of the International Union of Microbiological Society
T. Mizuki, M. Kamekura, S. DasSarma, T. Fukushima, R. Usami, Y. Yoshida and K. Horikoshi: Ureases of extreme halophiles of the genus Haloarcula with a unique structure of gene cluster
　Reproduced from Biosci. Biotechnol. Biochem., 68: 397-406 (2004) by copyright permission of the Japan Society for Bioscience, Biotechnology, and Agrochemistry
Y. Boucher, M. Kamekura and W. F. Doolittle: Origins and evolution of isoprenoid lipid biosynthesis in aechaea
　Reproduced from Mol. Microbiol., 52: 515-527 (2004) by copyright permission of the Blackwell Publishing Ltd
T. Mizuki, M. Kamekura, M. Ishibashi, R. Usami, Y. Yoshida, M. Tokunaga and K. Horikoshi: Nucleoside diphosphate kinase of halobacteria―Amino acid sequence and salt-response pattern
　Reproduced from J. Japn. Soc. Extremophiles, 3: 18-27 (2004) by copyright permission of the Japan Society for Extremophiles
H. Tokunaga, K. Mitsuo, M. Kamekura and M. Tokunaga: Major outer membrane proteins in moderately halophilic eubacteria of genera Chromohalobacter and Halomonas
　Reproduced from J. Basic Microbiol., 44: 232-240 (2004) by copyright permission of the John Wiley & Sons, Inc.
Y. Boucher, C. J. Douady, A. K. Sharma, M. Kamekura and W. F. Doolittle: Intragenomic heterogeneity and intergenomic recombination among haloarchaeal ribosomal RNA genes
　Reproduced from J. Bacteriol., 186: 3980-3990 (2004) by copyright permission of the American Society for Microbioligy
M. Akahira-Azuma, M. Szczepanik, R. F. Tsuji, R. A. Campos, A. Itakura, N. Mobini, J. McNiff, I. Kawikova, B. Lu, C. Gerard, J. S. Pober and P. W. Askenase: Early delayed-type hypersensitivity eosinophil infiltrates depend on T helper 2 cytokines and interferon-gamma via CXCR3 chemokines
　Reproduced from Immunology, 111: 306-317 (2004) by copyright permission of the Blackwell Publishing Ltd
T. Koga, P. Kwan, L. Zubik, C. Ameho, D. Smith and M. Meydani: Vitamin E supplementation suppresses macrophage accumulation and endothelial cell expression of adhesion molecules in the aorta of hypercholesterolemic rabbits
　Reproduced from Atherosclerosis, 176: 265-272 (2004) by copyright permission of the Elsevier Science Ltd
A. Oda, C. Sakuta, S. Masuda, T. Mizoguchi, H. Kamada and S. Satoh: Possible Involvement of Leaf Gibberellins in the Clock-Controlled Expression of XSP30, a Gene Encoding a Xylem Sap Lectin, in Cucumber Roots
　Reproduced from Plant Physiology, 133: 1779-1790 (2004) by copyright permission of the Springer-Verlag

Papers in Japanese

K. Matsushima, T. Takahashi, Y. Hanya and Y. Koyama: Molecular Biological study for Non-producibility of Aflatoxin by Koji mold Aspergillus sojae
　Reproduced from J. Japan Soysauce Research Institute, 30: 5-11 (2004) by copyright permission of the Japan Soysauce Research Institute
S. Hara, R. F. Tsuji, O. Hatamoto and T. Masuda: A Simple Method for Enrichment of Uninucleate conidia of Aspergillus oryzae
　Reproduced from J. Japan Soysauce Research Institute, 29: 229-234 (2003) by copyright permission of the Japan Soysauce Research Institute

Japanese Patents Laid-Open

Titles of Oral and Poster Presentations

III. Report on the Research Assisted by the NISR Research Grant in 2003

Research Grant

Harushi NAKAJIMA (The University of Tokyo): Cell surface engineering in Aspergillus oryzae
Hirofumi SHOUN (The University of Tokyo): Basic studies on fungal unique P450 for industrial applications
Masao TOKUNAGA (Kagoshima University): Development of highly soluble enzymes adopting the structural characteristics of halophilic enzymes
Masaaki WACHI (Tokyo Institute of Technology): Analysis of the role of E. coli RNA-binding protein Hfq in the regulation mechanism of gene expression
Yoh WADA (Osaka University): Modification vacuolar function and physiology of Saccharomyces cerevisiae by expressing mammalian V-ATPases

Young Investigator Research Grant

Shin HARUTA (The University of Tokyo): Microbial ecological studies on traditional food fermentation
Takane KATAYAMA (Kyoto University): Functional analysis of the proteins involved in the aromatic amino acids metabolism in bacteria
Jun-ichi MARUYAMA (The University of Tokyo): Functional characterization of AoHex1 involved in Woronin body formation in Aspergillus oryzae
Hiroyasu ONAKA (Toyama Prefectural University): Characterization of the biosynthetic gene cluster of goadsporin which promote secondary metabolism and sporulation in actinomycetes.
Yumiko TAKATSUKA (Tohoku University): Molecular dissection of the Selenomonas ruminantium cell envelope and lysine decarboxylase involved in the biosynthesis of a polyamine covalently linked to the cell wall peptidoglycan layer
Shinji TAKENAKA (Kobe University): Characterization of aerobic denitrification mechanism in Klebsiella pneumoniae strain F-5-2 that can utilize ammonium and nitrate ions simultaneously and application of nitrifying and denitrifying processes to wastewater treatment

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